Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA
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چکیده
منابع مشابه
High specificity of Trypanosoma cruzi epimastigote ribonucleoprotein as antigen in serodiagnosis of Chagas' disease.
We assessed the performance of an enzyme-linked immunosorbent assay (ELISA) with the Trypanosoma cruzi epimastigote ribosomal fraction (Tulahuen and Y strains) in order to improve the diagnostic specificity of the test. A total of 100 serum samples from patients with chronic Chagas' disease from Brazil and Argentina were studied. Sera from 116 patients, without Chagas' disease, including 10 wit...
متن کاملStructural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms.
Inositol phospholipids (IPL) from epimastigote forms of Trypanosoma cruzi have been investigated by metabolic labelling with [3H]palmitic acid and by GLC-MS analysis of the lipids obtained from non-labelled parasites. The IPL fraction was separated into phosphatidylinositol (PI) and inositol-phosphoceramide subfractions, the latter accounting for 80-85% of the total IPL. The neutral lipids rele...
متن کاملCharacterization of a Gi-protein from Trypanosoma cruzi epimastigote membranes.
A guanosine 5'-[gamma-[35S]thio]triphosphate-binding activity was detergent-extracted from Trypanosoma cruzi membranes. This binding activity was co-eluted from gel-filtration columns with a factor which, in a heterologous reconstitution system, blocks glucagon stimulation of adenylate cyclase activity in liver membranes. ADP-ribosylation of these membranes by pertussis toxin eliminated this bl...
متن کاملExperimental and Mathematical-Modeling Characterization of Trypanosoma cruzi Epimastigote Motility
The present work is aimed at characterizing the motility of parasite T. cruzi in its epimastigote form. To that end, we recorded the trajectories of two strains of this parasite (a wild-type strain and a stable transfected strain, which contains an ectopic copy of LYT1 gene and whose motility is known to be affected). We further extracted parasite trajectories from the recorded videos, and stat...
متن کاملTrypanosoma cruzi: Morphological and Microtubular Changes Related to Epimastigote Differentiation under Hyperosmotic Stress
Trypanosoma cruzi: Morphological and Microtubular Changes Related to Epimastigote Differentiation under Hyperosmotic Stress Alba Marina Gimenez1* and Estela E. Machado1 1Department of Molecular Biology, Faculty of Exact Sciences, Physical-Chemical and Natural Sciences, National University of Río Cuarto, Río Cuarto (X5800) Córdoba, Argentina SOJ Microbiology & Infectious Diseases Open Access Res...
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ژورنال
عنوان ژورنال: Revista do Instituto de Medicina Tropical de São Paulo
سال: 1994
ISSN: 0036-4665
DOI: 10.1590/s0036-46651994000200012